ARM-1 expression and adhesion to lung sections by cells treated with 4-HC. A. The effect of 4-HC on ARM-1 expression was evaluated by real time-PCR. 4-HC inhibited ARM-1 mRNA expression. Values are represented as ratios of ARM-1/GAPDH ± SD. B. B16-F10 cells were treated as described previously, and then incubated with lung sections during 30 min to allow their adhesion. The preparations were stained and photographed. Photomicrographs (400X) show that control cells (left) were able to adhere to lung sections; in contrast, 4-HC-treated cells (right) displayed impaired adhesion. C. Quantification of adhered cells (mean ± SE) from 5 independent experiments. On each experiment, 10 fields were counted. ***P < 0.0001 (Student's t test).