Figure 4

Loss of SFRP1 increases the migratory and invasive behavior of 76 N TERT cells. A, TERT-pSUPER and TERT-siSFRP1 cells were plated in 30 mm dishes and allowed to reach 100% confluence. A pipette tip was utilized to generate a wound (scratch) down the center of the plate. Image of the cells capable of migrating across the scratch 8 hours after the wound was created. Images were captured at 20× magnification; scale bar 100 μm. B, TERT-pSUPER and TERT-siSFRP1 cells that were plated in BD BioCoat™ control inserts or Matrigel™ invasion chambers and the cells capable of migrating through the 8 μm pore or invading the reconstituted basement membrane (Matrigel™) through the 8 μm pore towards a chemoattractant were stained with 10% crystal violet and counted. Images were captured at 10× magnification; scale bar 500 μm. (Experiments were repeated three times and the number of cells within a representative 10× field from each experiment were counted and bars represent mean ± SEM cell number. *p < 0.05,***p < 0.001 (significantly different from TERT-pSUPER cells using a student's t-test).