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Figure 3 | Cancer Cell International

Figure 3

From: Nucleic acid distribution pattern as a possible biomarker for metabolic activities of neoplastic cells: a digitally-aided fluorescence microscopy study on normal and neoplastic lymphocytes of acute and chronic canine lymphocytic leukemia

Figure 3

Wright-Giemsa-stained blood smear images (A, B), and fluorescence microscopy images (C, D) of canine acute lymphocytic leukemia cells (N197605x.tif; Capture_10-11-05, Capture_14-11-05). The smaller neoplastic lymphocytes in panel A are equivalent to the fluorescing images in panels C, while the image of the large neoplastic lymphocyte in panel B is equivalent to the fluorescing cell in panels D. Original images were acquired at 100 objective lens magnification. Bar = 6 μm. Note the diffuse green DSNA and red SSNA fluorescence within the nuclear regions, compared to the cytoplasmic region with only red SSNA fluorescence. The smaller neoplastic cells have high nuclear to cytoplasmic ratio compared to the larger cells. Erythrocytes are devoid of any nucleic acid fluorescence.

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