Figure 4

Higher magnification of fluorescence microscopy images of the neoplastic cells, showing small and large neoplastic cells. Original images were acquired at 100 objective lens magnification. Bar = 6 μm. Narrow cytoplasmic rim with processes laden with SSNA fluorescence are evident in the smaller neoplastic cells on the left. Cytoplasmic clumps of SSNA fluorescence are also quite evident in both cell-types. Nuclear fluorescence of the small neoplastic cells is characterized by diffuse yellow-green DSNA fluorescence with punctate, interconnected, channel-like, clumpy crimson-red SSNA fluorescence. Note the relative sizes of the small and large neoplastic cells, as well as the more oval outline of the nucleus of the large neoplastic cell which is also more eccentric, compared to the smaller cells. Nucleolar subdomains (yellow arrows) are evident in the large neoplastic cell. Non-fluorescing cisternal network (green arrows) is evident in the cytoplasm of the large cell.