Figure 5

Higher magnified fluorescence microscopy images, showing the large neoplastic cell and a normal appearing lymphocyte (Lower left). The insert represents equivalent large neoplastic cell prepared through routine Wright's stain, light microscopy method. Original images were acquired at 100 objective lens magnification. Bar = 6 μm. The nuclear region of the large neoplastic cell presents narrow clumps of dull green DSNA fluorescence and punctate areas of larger more diffuse clumps of red SSNA fluorescence. Nucleolar subdomain are shown as contiguous clumps of red SSNA fluorescence (Arrows). Non-fluorescing cisternal network, equivalent to the vesiculated pale structure in the Wright-stained image of the insert, is also evident in an eccentric area of the large neoplastic cell. Note the outline of the faint red SSNA fluorescence areas within the nuclear region of the lower left image of the normal-appearing lymphocyte; the distribution of which appears channel-like and interconnected. The normal-appearing cell is located close to the cytoplasmic area of the large neoplastic cell with non-fluorescing cisternal structures.