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Table 1 96 h cell lines grown in 10 mM glucose either with or without acetoacetate*

From: Acetoacetate reduces growth and ATP concentration in cancer cell lines which over-express uncoupling protein 2

Cell line
[Acetoacetate]d ATPe nmols/mg protein %ATPf Cell count ×106/ml %cell countg UCP2 OD % viability Total Protein/106 cells % total Protein
RFP3         
*0 85.2 100% 0.85 100% 66.0 99.7 9.85 100%
*10 mM 90.2 106% 0.805 94.7% 47.7 99.7 10.63 107.9%
MCH 064         
*0 146.3 100% 0.126 100% 104.5 94.0 7.61 100%
*10 mM 185.8 127% 0.140 111.1% 69.7 98.2 8.80 115.6%
MCH 065         
*0 198.5 100% 0.198 100% 80.0h 98.8 8.75 100%
*10 mM 184.5 93.2% 0.206 101.5% 59.9h 97.0 9.45 108.0%
SW48         
*0 693.5 100% 1.94 100% 177.7 99.4 7.8 100%
*10 mM 303.5 43.8% 0.76 39% 109.7 100 7.7 98.7%
SW480         
*0 597.0 100% 1.39 100% 182.7 95.5 12.8 100%
*10 mM 428.5 71.8% 0.79 57% 161.8 93.5 11.6 90.6%
RKO         
*0 700.0 100% 5.40 100% 221.1 100 14.6 100%
*10 mM 371.0 53% 2.74 50.7 181.5 100 12.6 86.3%
MCF-7         
*0 590.0 100% 0.87 100% 177.1 97.0 11.95 100%
*10 mM 540.5 91.6% 0.65 74.7% 148.2 91.5 10.8 90.5%
LOVO         
*0 433.0 100% 1.02 100% 150.5 98.9 13.1 100%
*10 mM 293.5 67.8% 0.60 58.7% 205.2 97.9 9.50 68.3%
CaCO2         
*0 296.5 100% 0.275 100% 224.7 93.5 7.10 100%
*10 mM 137.0 46.2% 0.140 50.9% 295.0 92.9 7.90 111.3%
MDA MB 231         
*0 267.0 100% 0.72 100% 556.7 99.1 17.95 100%
*10 mM 161.5 60.5% 0.34 47.6% 341.5 99.3 21.0 117.0%
  1. *For standard errors of all measurements see Figures 2 – 5 (based upon ATP and cell count studies performed in triplicate; and UCP2 measurements performed in duplicate).
  2. ATP and cell growth values in bold print are significantly lower (p < 0.05) than the values in glucose only medium for the same cell line. UCP2 values in bold italics are significantly higher (p < 0.05) than mean of control fibroblast lines for each growth medium.
  3. d [Acetoacetate] = Concentration of Acetoacetate in medium
  4. e ATP production expressed as nmol ATP/mg protein
  5. f % of ATP production at 96 h compared with 10 mM glucose and 0 mM acetoacetate (defined as 100%)
  6. g Cell count expressed as % of 96 h growth in 10 mM glucose and 0 mM acetoacetate
  7. h Calculated from the slope of its ELISA curve (0.043/μg protein, not shown) in comparison to Fig. 1B for RFP3 cells, then recalibrated to ImageJ OD/cell.
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