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Table 1 96 h cell lines grown in 10 mM glucose either with or without acetoacetate*

From: Acetoacetate reduces growth and ATP concentration in cancer cell lines which over-express uncoupling protein 2

Cell line

[Acetoacetate]d

ATPe nmols/mg protein

%ATPf

Cell count ×106/ml

%cell countg

UCP2 OD

% viability

Total Protein/106 cells

% total Protein

RFP3

        

*0

85.2

100%

0.85

100%

66.0

99.7

9.85

100%

*10 mM

90.2

106%

0.805

94.7%

47.7

99.7

10.63

107.9%

MCH 064

        

*0

146.3

100%

0.126

100%

104.5

94.0

7.61

100%

*10 mM

185.8

127%

0.140

111.1%

69.7

98.2

8.80

115.6%

MCH 065

        

*0

198.5

100%

0.198

100%

80.0h

98.8

8.75

100%

*10 mM

184.5

93.2%

0.206

101.5%

59.9h

97.0

9.45

108.0%

SW48

        

*0

693.5

100%

1.94

100%

177.7

99.4

7.8

100%

*10 mM

303.5

43.8%

0.76

39%

109.7

100

7.7

98.7%

SW480

        

*0

597.0

100%

1.39

100%

182.7

95.5

12.8

100%

*10 mM

428.5

71.8%

0.79

57%

161.8

93.5

11.6

90.6%

RKO

        

*0

700.0

100%

5.40

100%

221.1

100

14.6

100%

*10 mM

371.0

53%

2.74

50.7

181.5

100

12.6

86.3%

MCF-7

        

*0

590.0

100%

0.87

100%

177.1

97.0

11.95

100%

*10 mM

540.5

91.6%

0.65

74.7%

148.2

91.5

10.8

90.5%

LOVO

        

*0

433.0

100%

1.02

100%

150.5

98.9

13.1

100%

*10 mM

293.5

67.8%

0.60

58.7%

205.2

97.9

9.50

68.3%

CaCO2

        

*0

296.5

100%

0.275

100%

224.7

93.5

7.10

100%

*10 mM

137.0

46.2%

0.140

50.9%

295.0

92.9

7.90

111.3%

MDA MB 231

        

*0

267.0

100%

0.72

100%

556.7

99.1

17.95

100%

*10 mM

161.5

60.5%

0.34

47.6%

341.5

99.3

21.0

117.0%

  1. *For standard errors of all measurements see Figures 2 – 5 (based upon ATP and cell count studies performed in triplicate; and UCP2 measurements performed in duplicate).
  2. ATP and cell growth values in bold print are significantly lower (p < 0.05) than the values in glucose only medium for the same cell line. UCP2 values in bold italics are significantly higher (p < 0.05) than mean of control fibroblast lines for each growth medium.
  3. d [Acetoacetate] = Concentration of Acetoacetate in medium
  4. e ATP production expressed as nmol ATP/mg protein
  5. f % of ATP production at 96 h compared with 10 mM glucose and 0 mM acetoacetate (defined as 100%)
  6. g Cell count expressed as % of 96 h growth in 10 mM glucose and 0 mM acetoacetate
  7. h Calculated from the slope of its ELISA curve (0.043/μg protein, not shown) in comparison to Fig. 1B for RFP3 cells, then recalibrated to ImageJ OD/cell.
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Cancer Cell International

ISSN: 1475-2867

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