|
Cell line
|
|---|
|
[Acetoacetate]d
|
ATPe nmols/mg protein
|
%ATPf
|
Cell count ×106/ml
|
%cell countg
|
UCP2 OD
|
% viability
|
Total Protein/106 cells
|
% total Protein
|
|
RFP3
| | | | | | | | |
|
*0
|
85.2
|
100%
|
0.85
|
100%
|
66.0
|
99.7
|
9.85
|
100%
|
|
*10 mM
|
90.2
|
106%
|
0.805
|
94.7%
|
47.7
|
99.7
|
10.63
|
107.9%
|
|
MCH 064
| | | | | | | | |
|
*0
|
146.3
|
100%
|
0.126
|
100%
|
104.5
|
94.0
|
7.61
|
100%
|
|
*10 mM
|
185.8
|
127%
|
0.140
|
111.1%
|
69.7
|
98.2
|
8.80
|
115.6%
|
|
MCH 065
| | | | | | | | |
|
*0
|
198.5
|
100%
|
0.198
|
100%
|
80.0h
|
98.8
|
8.75
|
100%
|
|
*10 mM
|
184.5
|
93.2%
|
0.206
|
101.5%
|
59.9h
|
97.0
|
9.45
|
108.0%
|
|
SW48
| | | | | | | | |
|
*0
|
693.5
|
100%
|
1.94
|
100%
|
177.7
|
99.4
|
7.8
|
100%
|
|
*10 mM
|
303.5
|
43.8%
|
0.76
|
39%
|
109.7
|
100
|
7.7
|
98.7%
|
|
SW480
| | | | | | | | |
|
*0
|
597.0
|
100%
|
1.39
|
100%
|
182.7
|
95.5
|
12.8
|
100%
|
|
*10 mM
|
428.5
|
71.8%
|
0.79
|
57%
|
161.8
|
93.5
|
11.6
|
90.6%
|
|
RKO
| | | | | | | | |
|
*0
|
700.0
|
100%
|
5.40
|
100%
|
221.1
|
100
|
14.6
|
100%
|
|
*10 mM
|
371.0
|
53%
|
2.74
|
50.7
|
181.5
|
100
|
12.6
|
86.3%
|
|
MCF-7
| | | | | | | | |
|
*0
|
590.0
|
100%
|
0.87
|
100%
|
177.1
|
97.0
|
11.95
|
100%
|
|
*10 mM
|
540.5
|
91.6%
|
0.65
|
74.7%
|
148.2
|
91.5
|
10.8
|
90.5%
|
|
LOVO
| | | | | | | | |
|
*0
|
433.0
|
100%
|
1.02
|
100%
|
150.5
|
98.9
|
13.1
|
100%
|
|
*10 mM
|
293.5
|
67.8%
|
0.60
|
58.7%
|
205.2
|
97.9
|
9.50
|
68.3%
|
|
CaCO2
| | | | | | | | |
|
*0
|
296.5
|
100%
|
0.275
|
100%
|
224.7
|
93.5
|
7.10
|
100%
|
|
*10 mM
|
137.0
|
46.2%
|
0.140
|
50.9%
|
295.0
|
92.9
|
7.90
|
111.3%
|
|
MDA MB 231
| | | | | | | | |
|
*0
|
267.0
|
100%
|
0.72
|
100%
|
556.7
|
99.1
|
17.95
|
100%
|
|
*10 mM
|
161.5
|
60.5%
|
0.34
|
47.6%
|
341.5
|
99.3
|
21.0
|
117.0%
|
- *For standard errors of all measurements see Figures 2 – 5 (based upon ATP and cell count studies performed in triplicate; and UCP2 measurements performed in duplicate).
- ATP and cell growth values in bold print are significantly lower (p < 0.05) than the values in glucose only medium for the same cell line. UCP2 values in bold italics are significantly higher (p < 0.05) than mean of control fibroblast lines for each growth medium.
- d [Acetoacetate] = Concentration of Acetoacetate in medium
- e ATP production expressed as nmol ATP/mg protein
- f % of ATP production at 96 h compared with 10 mM glucose and 0 mM acetoacetate (defined as 100%)
- g Cell count expressed as % of 96 h growth in 10 mM glucose and 0 mM acetoacetate
- h Calculated from the slope of its ELISA curve (0.043/μg protein, not shown) in comparison to Fig. 1B for RFP3 cells, then recalibrated to ImageJ OD/cell.
