Nuclear staining of HepG2 cells with Hoecsht 33258. Cells were treated with eurycomanone and control drug, tamoxifen. (a) Untreated (control) HepG2 cells remained uniformly stained with round and unpunctuated nucleus. (b) Tamoxifen-treated HepG2 at 24 hours. (c) Tamoxifen-treated HepG2 at 48 hours. (d) Tamoxifen-treated HepG2 at 72 hours. (e) Eurycomanone-treated HepG2 at 24 hours. (f) Eurycomanone-treated HepG2 at 48 hours. (g) Eurycomanone-treated HepG2 at 72 hours. Eurycomanone-treated HepG2 cells showed apoptotic morphology; cell shrinkage, DNA condensation and nuclear fragmentation as for tamoxifen.