Immunohistochemical staining of DCIS and IDC with anti-NUCKS and anti-ki67 antibody. Tissue sections stained with anti-NUCKS (A-C) and corresponding sections stained with anti-ki67 (D-F) antibody. (A), (D), Strong immunoreaction for NUCKS and low for Ki67 in DCIS. (B), (E) Strong immunoreaction for NUCKS and low for Ki67 in IDC, grade II. (C), (F) IDC, grade III negative staining for NUCKS and strong staining for Ki67. Scale bar, 50 μm.