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Table 3 Primers and probes for semi-quantitative and real-time PCR

From: NUCKS overexpression in breast cancer

Oligonucleotide Sequence
NUCKS F (forward) 5'-AATTCACAGGAAGATAGTGAGG-3'
NUCKS R (reverse) 5'-TCACTGTAG CCTTTAGTCTGGG-3'
GAPDH forward 5'-ATGGGGAAGGTGAAGGTCGGAGTC-3'
GAPDH reverse 5'-GAACATGGGGGCGTCAGCAGAG-3'
NUCKS Fq (forward, real-time PCR) 5'-TCTGATGATGCAGATGAAGATTA-3'
NUCKS Rq (reverse, real-time PCR) 5'-CTGCTGAGTGAGAATCATCC-3'
NUCKS FL probe 5'-TCTTGGTCTTCACATCTTTGTCTTCT-FL-3'
NUCKS LC probe 5'-LC640-AGTCCTCACTATCTTCCTGTGAATTCTTT-3'