Scrape loading/dye transfer assay performed in BRL3A, HTC and HTC-Cx43. DIC images in A1, B1 and C1, fluorescence images in A2, B2 and C2, merge in A3, B3 and C3. BRL3A presented good communication capacity (at least 5 rows of fluorescent cells from scrape were observed (A). HTC and HTC-Cx43 were deficient in GJIC. Fluorescent cells were visualized only in areas near to scrape.