Expression of TRPC mRNA in breast cancer epithelial cell lines. (A) PCR with TRPC subtype specific primers was utilized to examine the expression of TRPC mRNAs in cDNA prepared from MCF-10A, MCF-7 and MDA-MB-231 cell lines. The PCR reactions were separated on an agarose gel. Molecular weight markers in base pairs are shown to the right of the gel. As a control for the integrity of each cDNA sample β-actin was also analysed. (B) Relative expression of TRPC subtype specific mRNAs in cDNA prepared from MCF-10A, MCF-7 and MDA-MB-231 cells. All data normalized to TRPC3 expression in MCF-7 cells.