Figure 4

Localization and co-localization of TRPC6 and TRPC3 in breast cancer epithelial cell lines. (A) Immunoflourescent confocal images of TRPC6 protein expression in MCF-7 and MDA-MB-231 cell lines. Cells were treated with a concanavalin-FITC conjugate to stain the plasma membrane before permeabilization and probing with a TRPC6 specific antibody. Scale bar represents 20 μM. (B) Immunoflourescent confocal images of TRPC3 protein expression in MDA-MB-231 cells. Cells were treated with a concanavalin-FITC conjugate to stain the plasma membrane before permeabilization and probing with a TRPC3 specific antibody. The TRPC3/6 and conacavalin-FITC images were merged to indicate co-localization of the plasma membrane with TRPC3/6 expression. Scale bar represents 20 μM. (C) Immunoflourescent confocal images to determine TRPC6 protein expression in MCF-10A cells. Cells were treated with a concanavalin-FITC conjugate to stain the plasma membrane before permeabilization and probing with a TRPC6 specific antibody. Scale bar represents 40 μM. (D) Co-localization of TRPC6 and TRPC3 in MDA-MB-231 cells. Images were merged to indicate co-localization of TRPC3 and TRPC6 antibody staining. Scale bar represents 32 μM.