Figure 4
L1-binding integrin expression. (A) 9L/LacZ cells were immunostained live with a mouse monoclonal antibody against integrin αvβ5 (green) and nuclei were counterstained with bisbenzimide (blue). Cell surface staining for integrin αvβ5 is clearly visible. (B) Rat glioma cells 9L/LacZ and C6/LacZ and human glioma cells U87/LacZ were immunostained live for integrin αvβ5 and analyzed by flow cytometry. All 3 cell lines exhibited distinct positive peaks for integrin αvβ5 (blue lines) compared to no primary antibody negative controls (red lines), and U87/LacZ cells appeared to have two overlapping positive populations: one high-level positive population and another lower-lever positive population.