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Figure 2 | Cancer Cell International

Figure 2

From: INTS6/DICE1 inhibits growth of human androgen-independent prostate cancer cells by altering the cell cycle profile and Wnt signaling

Figure 2

Inhibition of colony formation of PC3 and DU145 prostate cancer cells by pDICE1-EGFP fusion construct. PC3 and DU145 cells were transiently transfected with the pEGFP or pDICE1-EGFP fusion constructs. A. Equivalent transfection efficiencies (30-40%) at 48 hours post-transfection were verified by reporting EGFP in situ fluorescence on the total number of cells (regular light) using an inverted microscope (left). Expression of constructs was analyzed in PC3 cells by Western blotting of EGFP and DICE1-EGFP fusion protein using an anti-GFP monoclonal antibody (right). Sizes in kilodalton (kD) are indicated on the left. B. After transfection, prostate cancer cells were selected over 2-3 weeks in the presence of G418 antibiotic and stained with 0.5% crystal violet in methanol (left). Quantitative analysis of inhibition of the colony formation in PC3 and DU145 cells transfected with pEGFP and pDICE1-EGFP plasmid (right).

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