Table 2 Genes and corresponding primer sequences
Gene Symbol | Forward Primer 5' → 3' | Reverse Primer 5' → 3' | Amplicon size (bp) | Annealing Temp. (°C) |
|---|---|---|---|---|
G3PDH | TGGTATCGTGGAAGGACTCA | ATGCCAGTGAGCTTCCCGTT | 188 | 58 |
DICE1 | TGCCCATCTTACTGTTCCTG | TCTTCGAAAGTGACCAGC | 169 | 58 |
CXXC4 | TGCAAGAGGCTCATCAACTG | TCATTTCCAAATGCCTTGAA | 204 | 60 |
CCND1 | AGGAACAGAAGTGCGAGGAG | GGCGGATTGGAAATGAACT | 394 | 60 |
TCF7L1 | ACGAGCTGATCCCCTTCC | TGACCTCGTGTCCTTGACTG | 400 | 60 |