Figure 1

Effect of oxaliplatin on cholangiocarcinoma cells. (A) RMCCA1 and (B) KKU100 cells were treated with oxaliplatin at various concentrations (0, 10, 50, 100 and 200 μM) for 48 hours. Effect on cell proliferation was measured by WST-1 and analyzed by spectrophotometric analysis (Absorbance = 450 nm). Results are reported as percentage inhibition of cell proliferation, where the optical density value from vehicle-treated cells was set as 100% of proliferation. Results are represented by the mean ± SE of three independent experiments. Phosphorylation of Akt and mTOR in (C) RMCCA1 and (D) KKU100 cells following 0–200 μM oxaliplatin treatment for 12 h and phosphorylation of Akt and mTOR in (E) RMCCA1 and (F) KKU100 cells following 100 μM oxaliplatin treatments for 0–48 h were determined by western blotting. Total Akt and mTOR were used as loading control. Representatives of the three independent experiments are shown.