Figure 1
Growth inhibition and lethality of cisplatin towards OV2008, OV2008/C13, A2780 and A2780/CP70 cells. Cells were cultured in the presence of the indicated concentrations of cisplatin for 1 h and maintained in cisplatin-free medium for 3 days. (A) and (C), the total number of cells was recorded at the beginning of the experiment and after 3 days of treatment. The difference between number of cells in vehicle-treated controls at 0 h and after 3 days of culture was considered to be 100%. The growth of the treated groups is expressed as percentage of control. The experiment was repeated three times with different cell line passages to calculate the IC50 (see averaged data on Table 1). Depicted are representative experiments. (B) and (D), after treatment, viability was assessed by microcapillary cytometry. Results are the average of triplicate counts ± SEM. *p < 0.05, #p < 0.01 and †p < 0.001 vs. control (0 μM cisplatin) for the respective cell line.