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Figure 2 | Cancer Cell International

Figure 2

From: Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture

Figure 2

Morphology observed for MCF-10A, MCF-10AT, and MCF-10CA1a cells grown in rBM for 20 days. Phase contrast images of normal MCF-10A cells were cultured in overlay rBM for 2, 4, 8, 12, 16, and 20 days (top, A to F). Transformed MCF-10AT cells (middle, G to L) and malignant MCF-10CA1a cells (bottom, M to R) were cultured in three-dimensions at the same time points. Scale bar, 100 μm. Normal MCF-10A cells increase in size to day 8 and form polarized spherical acini. Transformed MCF-10AT cells increase in size for 20 days, retain polarity, and form multi-acinar structures. Malignant MCF-10CA1a cells continue to proliferate throughout the 20 days and form large, complex masses.

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Cancer Cell International

ISSN: 1475-2867

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