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Figure 5 | Cancer Cell International

Figure 5

From: Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture

Figure 5

Apoptosis observed for MCF-10A, MCF-10AT and MCF-10CA1a cells grown in three-dimensional culture. Single confocal sections of activated caspase-3 staining (left) and nuclear staining with Draq 5 (middle) with matching differential interference contrast images (DIC-right) of MCF-10A acini in overlay three-dimensional culture at day 6 (A-C), day 12 (J-L) and day 20 (S-U); MCF-10AT multi-acinar structures at day 6 (D-F), day 12 (M-O), and day 20 (V-X); and MCF-10CA1a structures at day 6 (G-I), day 12 (P-R), and day 20 (Y-AA). Scale bars, 50 μm. Activated caspase 3 positive cells were observed at day 12 and 20 for MCF-10A acini, were not observed for MCF-10AT multi-acinar structures and were observed at days 6, 12, and 20 for MCF-10CA1a masses. The speckled fluorescence outside the acini is from the Matrigel (panel S).

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Cancer Cell International

ISSN: 1475-2867

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