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Figure 7 | Cancer Cell International

Figure 7

From: Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture

Figure 7

Cadherin and Laminin V staining observed for MCF-10A, MCF-10AT, and MCF-10CA1a cells in three-dimensional culture. MCF-10A (top), MCF-10AT (middle), and MCF-10CA1a (bottom) cell lines stained to examine cell:cell junctions and basement membrane formation during growth in overlay three-dimensional culture for 20 days. Single confocal sections of cadherin (A, E, I) demonstrate the existence of cell: cell junctions for all three cell lines at day 20 in three-dimensional culture. Laminin V (B, F, J) staining indicates basement membrane deposition for all three cell lines. Nuclei were stained with Draq5 (C, G, K) and matching differential interference contrast images are shown (D, H, L). Scale bars, 50 μm.

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Cancer Cell International

ISSN: 1475-2867

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