Figure 4

Panel One: Incubation of MB361 cells with anti-MAM antibody and induction of cell apoptosis. Cell viability assay was conducted after incubation of MB361 breast cancer cells with anti-MAM. The number of the dead cells (in red color) was found higher in the anti-MAM incubated cancer cells (B), as compared with that in the non-incubated MB361 cells (A). There were no or few dead HAEC cells (C). The images were taken with objective lens 10×. Panel Two: Western blot assay for the pro-PARP-1 (100 kDa) and activated PARP-1 (89 kDa) in the protein lysates of MDA-MB361, T47D, and HAEC cells. The Con – control cells with no anti-MAM antibody inoculation, MAM – cells with anti-MAM antibody inoculation.