Figure 5

Specificity and cytotoxicity assay of the synthesized MAM targeted LDL particles. The images (A-C) show the cell binding and endocytosis of free Dox, LDL-Dox, and anti-MAM-LDL-Dox. Obvious Dox uptake was found in HAEC cells when the cells were incubated with free Dox and LDL-Dox (Dox is red fluorogenic), but not in the cells incubated with the anti-MAM-LDL-Dox indicating that conjugation of anti-MAM blocks the binding sites of LDL to LDLR. The LDL-Dox and free Dox incubations are served as positive controls in this experiment. The images (D-F) are matched phase images (A-C). The images (G-I) show the results of the cell viability assays after incubation with free Dox, LDL-Dox, and anti-MAM-LDL-Dox, many dead cells (red color) were found when the HAEC cells were incubated with free Dox and LDL-Dox, but few dead cells were shown when they were incubated with anti-MAM-LDL-Dox (most of the cells are green). The images (J-L) show the cytotoxicities of free Dox, LDL-Dox and Anti-MAM-LDL-Dox to MB415 Cells. As shown, most of the cells were dead (small and red) when they were treated by free Dox and anti-MAM-LDL-Dox; while only a few dead cells were found dead when they were treated by LDL-Dox. The images were taken with objective lens 10×.