Type of PEG used for pegylation | rhArg1: PEG ratio (mol/mol) for pegylation | Relative arginase activity (%) |
---|---|---|
Native rhArg1 | --- | 100 |
mPEG-SPA | 1:20 | 94 |
 | 1:50 | 93 |
mPEG-NHS | 1:20 | 86 |
 | 1:50 | 83 |
mPEG-MAL | 1:20 | 85 |
 | 1:50 | 84 |
mPEG-ALD | 1:20 | 88 |
 | 1:50 | 88 |
mPEG-CN | 1:20 | 34 |
 | 1:50 | 30 |