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Table 1 rhArg1 was reacted with five different PEGs, varying in linker chemistry.

From: Pegylated derivatives of recombinant human arginase (rhArg1) for sustained in vivo activity in cancer therapy: preparation, characterization and analysis of their pharmacodynamics in vivo and in vitro and action upon hepatocellular carcinoma cell (HCC)

Type of PEG used for pegylation

rhArg1: PEG ratio (mol/mol) for pegylation

Relative arginase activity (%)

Native rhArg1

---

100

mPEG-SPA

1:20

94

 

1:50

93

mPEG-NHS

1:20

86

 

1:50

83

mPEG-MAL

1:20

85

 

1:50

84

mPEG-ALD

1:20

88

 

1:50

88

mPEG-CN

1:20

34

 

1:50

30

  1. Different forms of pegylated rhArg1 were prepared, purified and analyzed for their specific activities. Relative arginase activity indicates the specific activity of pegylated rhArg1 with respect to native rhArg1.