Figure 1

Proteomic pattern (IEF of pI 3-10 and pI 4-7 followed by 12.5% SDS-PAGE) and immuno-blotting profiles of membranous and cytoplasmic part of HepG2 (1^stAb: Hep88 mAb, dilution 1: 100; 2^ndAb: rabbit anti-mouse Ab, dilution 1: 3,000; Detection system: alkaline phosphatase & BCIP/NBT). (A-B) Coomassie blue staining of 2-D gel of membrane proteins with IEF of pI 3-10 and pI 4-7, respectively. (C-D) Coomassie blue staining of 2-D gel of cytoplasmic proteins with pI 3-10 and 4-7, respectively. (E-F) Immuno-staining of membrane proteins with Hep88 mAbwith pI 3-10 and 4-7, respectively. (G-H) Immuno-staining of cytoplasmic proteins with Hep88 mAb with pI 3-10 and 4-7, respectively. Protein spots which were further analyzed by LC-MS were indicatedby red arrows.