Figure 3
From: MiR-133b regulates bladder cancer cell proliferation and apoptosis by targeting Bcl-w and Akt1
Bcl-w and Akt1 as target genes of miR133b. (a) Schematic diagram of the predicted miR-133b binding site in the Bcl-w 3′-UTR. (b) Luciferase reporter assays in untreated HEK-293 T cells, or cotransfected with wild type (wt) or mutant (mut) Bcl-w 3′-UTR, and miR-133b or miR-133b negative control (NC). *P < 0.01 (c) Schematic diagram of the predicted miR-133b binding site in the Akt1 3′-UTR. (d) Luciferase reporter assays in untreated HEK-293 T cells, or cotransfected with wild type (wt) or mutant (mut) Akt1 3′-UTR, and miR-133b or miR-133b negative control (NC). *P < 0.01.