Figure 5

Myricetin enhances the 5-FU effection on alteration of the genes expression. EC9706 cells seeded in six-well plates were treated with myricetin (50 μM), 5-FU (80 μM) and myricetin +5-FU combination. After 48 h, total protein was extracted for western-blot. The relative expression level of Survivin, Cyclin D, Bcl-2, Caspas-3 and P53 from each independent experiments are shown. More alteration changes of these protein expression level were observed in myricetin and 5-FU combination group. Which indictated that myricetin in combination with 5-FU could promote the chemosensitivity of 5-FU in EC9706 cells by regulating the expression of Survivin, Cyclin D and Bcl-2, Caspase-3 and P53 (*P < 0.05 vs control, # P < 0.05 vs 5-FU). Data are presented as the mean of triplicate experiments.