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Figure 2 | Cancer Cell International

Figure 2

From: hsa-mir-30c promotes the invasive phenotype of metastatic breast cancer cells by targeting NOV/CCN3

Figure 2

The 5’-end of the hsa-mir-30c hairpin is the predominant mature miRNA detected in and promoting the invasiveness of MDA-MB-231 cells. (A) Representative images from Matrigel invasion assay following 48 h of transient transfection of siRNA of cells stained with HEMA3. (B) Percent of cells that migrated through the control inserts, defined by count of stained cells on the bottom of the inserts as a percent of cells loaded into inserts. Mean and SEM (error bars) for four technical replicates each of two biological replicates. (C) Percent of cells that invaded through the Matrigel inserts, normalized to the number of cells that migrated through the control inserts. Mean with SEM (error bars) for four technical replicates each of two biological replicates. * = Wilcoxon Rank Sum p-value < 0.01. (A-C) NT = non-targeting miRNA, 30c = hsa-mir-30c, and 30c* = hsa-mir-30c-3p. (D) qPCR detection of endogenous hsa-mir-30c (hsa-mir-30c-5p) and hsa-mir-30c* (hsa-mir-30c-3p) in MCF-7 and MDA-MB-231 cells. Mean with SEM (error bars) for two technical replicates each of two biological replicates normalized to U6 snRNA using the delta-Ct method. * = p-value from Student’s t-test with Welch correction comparing hsa-mir-30c (hsa-mir-30c-5p) and hsa-mir-30c* (hsa-mir-30c-3p) in MDA-MB-231 cells < 0.05.

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