Predictive, ontological, and qPCR screen for hsa-mir-30c reveals NOV as a target of hsa-mir-30c. (A) The log2 of the fold change in mean with SEM detection levels (hsa-mir-30c/non-targeting miRNA) is plotted for each set of primers for each transcript normalized to HPRT using delta-delta Ct method . (B) Representative Western blots for lysates extracted from MDA-MB-231 cells following 48 h of transient transfection with either non-targeting miRNA (NT) or hsa-mir-30c (30c). Top blot: NOV, bottom blot: Lamin C. (C) qPCR for NOV levels for two technical replicates each for three biological replicates following 48 h transient transfections of non-targeting miRNA (NT) or hsa-mir-30c (30c) normalized to HPRT using delta-delta Ct method . Mean with SEM. ** = p-value from Student’s t-test with Welch correction < 0.01. (D) Alignment of hsa-mir-30c (top sequences) with the 3’-UTR of NOV (bottom sequences) with the 5’-positions within the NOV 3’-UTR being relative to the 5’-start of the 3’-UTR for each of the three predicted targeting sites. Target scores are provided by mirSVR. Uppercase letters linked with a “|” character indicates a perfect match, while uppercase letters linked with a “:” character indicates a wobble pair.