Table 1 Genes chosen for qPCR screen
From: hsa-mir-30c promotes the invasive phenotype of metastatic breast cancer cells by targeting NOV/CCN3
Predicted hsa-mir-30c targets from microRNA.org | ||
|---|---|---|
Gene symbol | mirSVR Score | References into function or ontology terms |
TWF1 | −3.02 | GO: Actin Binding |
Regulates actin filament turnover [31] | ||
Promotes cellular motility through actin regulation [32] | ||
DYNLT3 | −3.02 | GO: Cytoplasm, GO: Plasma Membrane |
Cytoplasmic dynein light chain protein that interacts with spindle checkpoint protein BUB3 [33] | ||
Functions as a nuclear matrix-associated transcription factor in a dynein-independent manner [34] | ||
NEDD4 | −2.47 | Modulates p-Smad1 signaling in response to both BMP-2 and TGFβ1 [35] |
Negatively regulates PTEN in an oncogenic fashion [36] | ||
Involved in the deposition of extracellular collagen [37] | ||
PTPN3 | −2.55 | Regulates EGF-mediated cell-cell contact [38] |
Regulates focal adhesion [39] | ||
Regulates Cadherin-mediated cell-cell contact [40] | ||
Regulates neural crest cell adhesion and motility [41] | ||
Controls hormone receptor signaling [42] | ||
Cooperates with vitamin D to promote breast cancer cell growth [43] | ||
ADAM22 | −2.44 | GO: Extracellular, GO: Metallopeptidase Activity |
Metalloproteinase-like, disintegrin-like, cysteine-rich protein that is highly expressed in brain tissue [44] | ||
Promotes cell adhesion and spreading [45] | ||
Cooperates with SRC-1 in endocrine resistance of breast cancer cells; expression of ADAM22 independently predicts poor survival in patients [46] | ||
NOV | −3.11 | Regulates actin cytoskeletal reorganization [28] |
Increases motility of chondrosarcoma cells [47] | ||
Promotes mineralization of osteoblasts [48] | ||
Transcriptionally activated by p53 [24] | ||
Downregulated in advanced melanomas [26] | ||
Downregulated in childhood adrenocortical tumors [25] | ||
Significantly over-expressed in ER-positive breast cancer patients who relapsed after tamoxifen treatment [49] | ||
Expression is negatively correlated with metastasis and progression in breast cancer [50] | ||
Promotes xenograph breast cancer bone-metastasis and osteolysis [27] | ||
CELSR3 | −4.03 | GO: G-protein coupled receptor signaling pathway, GO: neuron migration, and GO: plasma membrane |