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Table 1 Genes chosen for qPCR screen

From: hsa-mir-30c promotes the invasive phenotype of metastatic breast cancer cells by targeting NOV/CCN3

Predicted hsa-mir-30c targets from
Gene symbol mirSVR Score References into function or ontology terms
TWF1 −3.02 GO: Actin Binding
Regulates actin filament turnover [31]
Promotes cellular motility through actin regulation [32]
DYNLT3 −3.02 GO: Cytoplasm, GO: Plasma Membrane
Cytoplasmic dynein light chain protein that interacts with spindle checkpoint protein BUB3 [33]
Functions as a nuclear matrix-associated transcription factor in a dynein-independent manner [34]
NEDD4 −2.47 Modulates p-Smad1 signaling in response to both BMP-2 and TGFβ1 [35]
Negatively regulates PTEN in an oncogenic fashion [36]
Involved in the deposition of extracellular collagen [37]
PTPN3 −2.55 Regulates EGF-mediated cell-cell contact [38]
Regulates focal adhesion [39]
Regulates Cadherin-mediated cell-cell contact [40]
Regulates neural crest cell adhesion and motility [41]
Controls hormone receptor signaling [42]
Cooperates with vitamin D to promote breast cancer cell growth [43]
ADAM22 −2.44 GO: Extracellular, GO: Metallopeptidase Activity
Metalloproteinase-like, disintegrin-like, cysteine-rich protein that is highly expressed in brain tissue [44]
Promotes cell adhesion and spreading [45]
Cooperates with SRC-1 in endocrine resistance of breast cancer cells; expression of ADAM22 independently predicts poor survival in patients [46]
NOV −3.11 Regulates actin cytoskeletal reorganization [28]
Increases motility of chondrosarcoma cells [47]
Promotes mineralization of osteoblasts [48]
Transcriptionally activated by p53 [24]
Downregulated in advanced melanomas [26]
Downregulated in childhood adrenocortical tumors [25]
Significantly over-expressed in ER-positive breast cancer patients who relapsed after tamoxifen treatment [49]
Expression is negatively correlated with metastasis and progression in breast cancer [50]
Promotes xenograph breast cancer bone-metastasis and osteolysis [27]
CELSR3 −4.03 GO: G-protein coupled receptor signaling pathway, GO: neuron migration, and GO: plasma membrane
  1. Genes were chosen for qPCR screen based on: mirSVR score ( [21], NCBI GeneRIF [22], and ontological terms [23]. TWF1 references: [31],[32]. DYNLT3 references: [33],[34]. NEDD4 references: [35]–[37]. PTPN3 references: [38]–[43]. ADAM22 references: [44]–[46]. NOV references: [24]–[28],[47],[48],[50].