The influence of CCN1 siRNA on U937 and Kasumi-1 cells. (A, B) CCK-8 reagent was used to measure AML cell proliferation. h, hour. (C) Cells were plated in 6-well plates (2000 cells/well), and the colonies were counted after 7 days. (D) Western blotting in AML cells treated with CCN1 siRNA. β-actin served as the loading control. (E) The cells were treated with cytarabine (2 μmol/L) for 18 hours, stained with Annexin V-APC/PI and analyzed by flow cytometry. **p < 0.01.