The MEK/ERK pathway was involved in CCN1 activity in AML. (A) Western blotting to determine the influence of CCN1 siRNA on β-catenin, MEK and ERK. Histone H1 served as the loading control for nuclear protein. (B) Western blotting to illustrate the influence of a 3-hour incubation with the CCN1 antibody on MEK and ERK. (C) Western blotting to determine the influence of a MEK/ERK inhibitor (PD98059, 20 μmol/L) on c-Myc, Bcl-xL and Bax. β-actin served as the loading control. (D) Schematic of the proteins related to CCN1 in AML cells. p-: phospho-.