Figure 3

Chemotactic invasion assay with AGAR - FBS spot. On the left (A), Hs 578T control cells into agar spot supplied with Fetal Bovine Serum (FBS). On the right (B), is reported the maximum distance from the border (d = 0.140 mm) reached by control cells, in the spot radius direction. On the left (C) Hs 578T control cells on the border of AGAR spot without FBS. On the right (D) are reported Hs 578T cells relative to five days of 5 mM K:D-rib treatment: three pre-treatment days and two treatment days during the chemotactic assay. On the left (E) are reported Hs 578T cells relative to seven days of 5 mM K:D-rib treatment: five pre-treatment days and two treatment days during the chemotactic assay. On the right (F) are reported Hs 578T cells relative to the seven day K:D-rib treatment: seven pre-treatment days and two days of chemotactic assay without treatment. On the left (G) are reported Hs 578T cells relative to nine days of 5 mM K:D-rib treatment: seven pre-treatment days and two treatment days during the chemotactic assay. On the right (H), is reported the maximum distance from the border (d = 0.11 mm) reached by cells, in the spot radius direction. The white line delimits the edge of the agar - FBS spot.