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Table 3 CDKN2A status evaluated with the use of real-time PCR at the DNA level

From: Astrocytoma-associated antigens - IL13Rα2, Fra-1, and EphA2 as potential markers to monitor the status of tumour-derived cell cultures in vitro

Sample

G108

G111

G112

G113

G114

Tumour

DEL (0.43)

DEL (0.17)

DEL (0.52)

- (0.86)

DEL (0.35)

1-2 months culture

DEL (0.67)

DEL (0.35)

- (1.22)

- (1.32)

DEL (0.05)

3-4 months culture

DEL (0.76)

DEL (0.00)

DEL (0.64)

- (0.89)

DEL (0.00)

5-7 months culture

DEL (0.54)

DEL (0.02)

DEL (0.00)

- (0.95)

n.a.

  1. Results are presented as relative gene dosage levels in comparison to control (values in brackets). Values ≤ 0.85 were considered as detection of a cell population demonstrating CDKN2A deletion (details in Methods section). DEL = deletion; n.a. = non-analysed.