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Table 4 EGFR gene dosage status evaluated with the use of real-time PCR

From: Astrocytoma-associated antigens - IL13Rα2, Fra-1, and EphA2 as potential markers to monitor the status of tumour-derived cell cultures in vitro

Sample

G108

G111

G112

G113

G114

Tumour

A/P (18.50)

A (6.18)

A (9.25)

Low A(1.78)

Low A (2.02)

1-2 months culture

Low A/P (3.58)

- (1.12)

Low A(1.69)

- (1.00)

Low A (3.57)

3-4 months culture

Low A/P (2.24)

Low A (1.58)

- (1.02)

- (1.31)

Low A (2.97)

5-7 months culture

Low A/P (1.85)

Low A (1.62)

Low A (1.68)

Low A(1.83)

n.a.

  1. Results are presented as relative EGFR gene dosage levels in comparison to control (values in brackets). Values ≥ 5 were considered as EGFR amplification (A); values 1.5-5 were considered as low amplification level of EGFR (low A). Polysomy 7 (P) were examined based on GPER gene dosage status (details in Methods section). n.a. = non-analysed.