Changes of T-lymphocyte subpopulation and differential expression pattern of the T-bet and GATA-3 genes in diffuse large B-cell lymphoma patients after chemotherapy

Table 3 Differential gene expression in DLBCL patients before and after chemotherapy

	EGR-1		T-bet		GATA-3		TGF-β
	ΔΔCt¹	2^-ΔΔCt	ΔΔCt¹	2^-ΔΔCt	ΔΔCt¹	2^-ΔΔCt	ΔΔCt²	2^-ΔΔCt
Before chemotherapy	−1.427 ± 0.565	2.67 ± 1.48	−3.236 ± 1.667	9.43 ± 2.14	−4.388 ± 0.839	20.83 ± 9.05	1.001 ± 0.943	2.01 ± 0.51
After three cycles of chemotherapy	1.117 ± 0.644	0.44 ± 0.23	−0.987 ± 0.606	1.98 ± 1.10	−2.517 ± 0.796	5.71 ± 1.62	0.314 ± 0.268	1.24 ± 0.57
p	0.001*		0.002*		0.028*		0.095

Note: ΔΔCt¹ = ΔCt _{target gene in control}-ΔCt _{target gene in patients}, 2^-ΔΔCt1 indicates the fold decrease in the expression of target genes in patients compared to controls. ΔΔCt² = ΔCt _{TGF-β in patient}-ΔCt _{TGF-β in control,} 2^-ΔΔCt2 indicates the fold increase in TGF-β expression in patients compared to controls. *indicates that there is significant difference between two groups.

ISSN: 1475-2867