Expression of CXCL1 in HCC tissue samples and HCC cell lines. (A) Determining the mRNA expression of CXCL1 in 48 cases of HCC tissues and corresponding nontumor tissues. (B) CXCL1 mRNA levels was measured by qRT-PCR as indicated HCC cell lines. (C) The protein expression levels of CXCL1 were determined by Western blot analysis in HCC cell lines. GAPDH was used as a loading control *P < 0.05, **P < 0.01.