The effect of CXCL1 on cell proliferation and invasion in 7721 and 7402 cell lines. (A) CXCL1 and p-P65 expression in the control and CXCL1-transfected cells was determined by Western blotting. GAPDH was used as a loading control. (B and C) Cell proliferation of CXCL1 was assessed in 7721 and 7402 cells by MTT at the indicated time. (D) Representative images of cells with or without CXCL1 transfection that had migrated into the lower chamber were shown. Quantitative analysis of cell invasion was assessed by invasion assay.