Metastatic poorly differentiated adenocarcinoma. (a - f) Histology and expression of caspase-3 by immunoperoxidase. (a - b and c - d) Treated and untreated tumors show similar morphological changes, H&E, 10× and 40×, respectively. (c and f) Caspase-3 demonstrates a mildly increased staining (black arrow) in treated tumor (4%) compared to (2%) in untreated tumor, immunoperoxidase ( IP), 40×. (g - h) HPLC runs of intracellular caspase-3 activity in treated and untreated tumor (AMC peak retention time, ~4.8 min).