Molecular changes in esophageal cancer cells co-cultured with hUCMSCs. The expressions of Bcl-2, Bax, survivin, MMP-2, MMP-9 in Eca109 cells or TE-1 cells were detected by qRT-PCR and Western blotting. Quantitative analysis showed that co-cultured Eca109 cells with hUCMSCs by transwell chamber significantly increased mRNA (A) levels and protein (C) levels of Bcl-2, survivin, MMP-2 and MMP-9. TE-1 cells co-cultured with hUCMSCs resulted in increased Bcl-2, survivin and MMP-2 mRNA (A) levels and protein (C) levels as compared to controls. (B) Representative Western blotting images. The results were the means ± SD of three independent experiments (*P < 0.05 versus control).