UA affects ΔΨ m in GBC cells. GBC-SD and SGC-996 cells were treated with UA for 48 h and then stained with the membrane-sensitive probe Rhodamine 123. (A) Rhodamine retention was measured by flow cytometry. The results are representative of 3 independent experiments. (B) The corresponding linear diagram shows the percentages of Rhodamine 123-negative cells as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. the control group.