Knockdown of β1 integrin expression by β1 siRNA in MCF-7-MT1 cells. Integrin β1 and control siRNAs were transfected into MCF-7-MT1 cells. (A): RNA was collected after 24 hours transfection and analyzed for β1 integrin expression by qRT-PCR. (B): 72 hours after transfection, cells were analyzed by flow cytometry for α2, α3, αV and β1 integrin. Control panel depicts cells stained with secondary antibody alone.