Figure 5
Downmodulation of NCR and NKG2D in NK-92 cells treated with the supernatant of HeLa, SiHa, and C-33A Cervical cancer cells (CCC). Supernatant of CCC was added to a final concentration of 30% in NK-92 cells. Then, the cells were incubated for 24 h at 37°C in a humid atmosphere containing 5% CO2 and 95% air in RPMI-S medium. Afterward, the expression of NCR or NKG2D was analyzed by Flow cytometry (FC). Values of percentage of expression and geometric Mean fluorescence intensity (MFI) for NKp30, NKp44, NKp46, and NKG2D in NK-92 cells treated or not with the supernatant of HeLa, SiHa, and C-33A CCC. Results are represented as the mean ± Standard deviation (SD) of three independent experiments performed in triplicate. *P <0.05 HeLa, SiHa, and C-33A vs. basal group, or HeLa, SiHa vs. basal group, Mann-Whitney U test.