Figure 1

Primary culture establishment from fresh tumor tissues of 30 HCC patients. Experimental protocol for establishing primary HCC culture from 30 patients was illustrated. Fresh tumor tissues were collected from HCC patients and underwent enzymatic digestion by type IV collagenase to release disaggregated cells. (A) For cases #1-20, cells were resuspended in AMEM supplemented with 10% FBS and then seeded onto 6-well culture plates coated with either 0.1% gelatin or 0.1% collagen. Phase contrast microscopy images of cultured cells from cases #14 and #17 were shown. (B) For cases #21-30, cells were resuspended in either AMEM supplemented with 10% FBS or HCM and then seeded onto 6-well culture plates coated with 0.1% gelatin. Phase contrast microscopy images of cultured cells from cases #21 and #29 were shown.