Phenotypic characterization of HCC21 cells. (A) Albumin levels of freshly isolated cells from patient’s tumor specimen, HCC21 cells at passage 1 and 6 were measured by flow cytometry. Percentages of albumin + cells were indicated in the histograms. (B) Phase-contrast microscopy images of cultured HCC21 cells at passages 6 and 30. (C) Growth curve of HCC21 cells at passage 10. (D) Wound healing assay showing the migration ability of HCC21 cells at passage 10.