Metastatic potential of HCC21 cells in NOD/SCID mice. (A) Subcutaneous injection of HCC21 cells into NOD/SCID mice led to primary tumor development at the site of injection and secondary tumors in the peritoneal cavity (scale bar = 10 mm). (B) Histological analysis of patient’s tumor and adjacent non-tumor liver tissues, primary and secondary xenograft tumors established in NOD/SCID mice. Sections were stained with haematoxylin and eosin (H&E) (upper panel), AFP (middle panel) and rabbit Ig control (lower panel). (C) Undetectable protein expression of E-cadherin in HCC21 (passage 12) while HepG2 served as positive control.