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Figure 5 | Cancer Cell International

Figure 5

From: Dynamic and influential interaction of cancer cells with normal epithelial cells in 3D culture

Figure 5

Expression of monocarboxylate transporters in a co- culture of MDA- MB -231 and MDCK cells and a monoculture of MCF7 in 2D and 3D. (A,B) MDA-MB-231 cells cultured alone in 3D, stained with anti-GFP (green, A) and anti-MCT1 (red, B) antibodies and with DAPI to visualize the nuclei (blue, A). The majority of MDA-MB-231 cells express MCT1, mostly on the cell membrane. (C,D) MDCK cells cultured alone in 3D, stained with anti-MCT1 (red, D) antibody and DAPI to visualize the nuclei (blue, C). The MDCK cells express MCT1, mainly on the cell membrane. (E,F) A co-culture of MDA-MB-231 and MDCK cells (1:1 ratio) in 3D, stained with anti-GFP (green, E) and anti-MCT1 (red, F) antibodies and with DAPI to visualize the nuclei (blue, E). E shows a mixed population of MDA-MB-231 (green) and MDCK cells in the sphere (no green). MCT1 is barely detectable in either cell types. (G,H) MCF7 cells cultured alone in 2D, stained with anti-GFP antibody labeling MCF7 cells (green, G), anti-MCT4 antibody (red, H) and DAPI to visualize the nuclei (blue, G). The MCF7 cells do not express MCT4 when cultured alone in 2D. (I,J) MCF7 cells cultured alone in 3D, stained with anti-GFP antibody for MCF7 (green, I), anti-MCT4 antibody (red, J) and DAPI to visualise the nuclei (blue, I). MCF7 cells strongly express MCT4 on their cell membrane when cultured in 3D. Scale bars; 50 μm.

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