The effects of phenoxodiol on the cell cycle of prostate cancer cell lines

Table 1 Primer sequences, product size, and annealing temperature

Gene	Sequence	Product size (base pairs)	Annealing temperature
AIF	Forward: GATCACGCTGTTGTGAGTGG	179 bp	61°C
AIF	Reverse: TCTTGTGCAGTTGCTTTTGC	179 bp	61°C
β-Catenin	Forward: GATTTGATGGAGTTGGAC	218 bp	52°C
β-Catenin	Reverse: TGTTCTTGAGTGAAGGAC	218 bp	52°C
Bax	Forward: GCTGGACATTGGACTTCCTC	167 bp	61°C
Bax	Reverse: TCAGCCCATCTTCTTCCAGA	167 bp	61°C
Bcl-xL	Forward: ACAATGCAGCAGCCGAGAG	167 bp	61°C
Bcl-xL	Reverse: ATGTGGTGGAGCAGAGAAGG	167 bp	61°C
Caspase 3	Forward: AAGGATCCTTAATAAAGGTATCCATGGAGAACACT	322 bp	55°C
Caspase 3	Reverse: AAAGAATTCCATCACGCATCAATTCCACAATTTCTT	322 bp	55°C
Cyclin-D1	Forward: AACTACCTGGACCGCTTCCT	165 bp	62°C
Cyclin-D1	Reverse: CCACTTGAGCTTGTTCACCA	165 bp	62°C
GAPDH	Forward: CAGAACATCATCCCTGCATCCACT	185 bp	60°C
GAPDH	Reverse: GTTGCTGTTGAAGTCACAGGAGAC	185 bp	60°C
Ki-67	Forward: AGTCAGACCCAGTGGACACC	225 bp	60°C
Ki-67	Reverse: TGCTGCCGGTTAAGTTCTCT	225 bp	60°C
L19	Forward: CTGAAGGTCAAAGGGAATGTG	194 bp	52°C
L19	Reverse: GGACAGAGTCTTGATGATCTC	194 bp	52°C
p21 ^WAF1	Forward: CCGAAGTCAGTTCCTTGTGG	333 bp	61°C
p21 ^WAF1	Reverse: AAGTCGAAGTTCCATCGCTCA	333 bp	61°C
sFRP4	Forward: CGATCGGTGCAAGTGTAAAA	181 bp	56°C
sFRP4	Reverse: GACTTGAGTTCGAGGGATGG	181 bp	56°C
XIAP	Forward: GGGGTTCAGTTTCAAGGACA	183 bp	56°C
XIAP	Reverse: CGCCTTAGCTGCTCTTCAGT	183 bp	56°C

ISSN: 1475-2867