Figure 2

The octamer element is important but not essential for non-B cell-derived Ig gene transcription. (A) Schematic diagram of mutated 5′ deletion truncations of the 1.2-kb pGL3 construct with a 4-bp deletion in the octamer motif (AGGCAAAT). (B) The intact and mutated 5′ deletion truncations of the 1.2-kb pGL3 construct were transfected into HeLa, HeLa MR, HeLa S3, HT-29, MDA-MB-231, HEK 293, L02, HK2, and Raji cells. Luciferase activity was measured using a dual-luciferase reporter system. The results are representative of three independent experiments after normalization to renilla luciferase activity (internal controls). Each bar represents mean ± SD.