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Figure 2 | Cancer Cell International

Figure 2

From: Upregulation of death receptor 5 and activation of caspase 8/3 play a critical role in ergosterol peroxide induced apoptosis in DU 145 prostate cancer cells

Figure 2

The effect of EP inducing apoptosis in DU 145 cells by cell cycle and TUNEL assay. (a) The effect of EP on sub G1 population in DU 145 cells. DU 145 prostate cancer were exposed to with EP (25 and 50 μM) for 24 h and stained with propidium iodide (50 μg/ml) for 30 min at room temperature in dark. Then flow cytometric analysis was performed. Data are presented as means ± S.D of triplicate samples. (b) Effect of EP on TUNEL assay in DU 145 cells. DU 145 cells treated with EP (25 and 50 μM) were seeded after fixing with 4% paraformaldehyde for 30 min and incubated in 25 ml of TUNEL assay mixture for 60 min at 37°C in the dark. TUNEL-stained cells were counter-stained with propidium iodide (Sigma, St. Louis, MO, USA) and visualized at x200 magnification by fluorescence microscopy.

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