Nucleofection optimization and validation of TRAIL gene and protein expression in ADMSCs. Optimization was performed based on different concentration of TRAIL encoded pLOC plasmid and cell number. Higher amount of the plasmid significantly increased percentage of efficiency and yield, with reduction on cells viability (A). However, increased total number of cells with the highest plasmid concentration enhanced cellular viability of the nucelofected ADMSCs (A). A substantially high level of TRAIL mRNA (B) and secreted protein expression (C) validated exogenous TRAIL expression in nucleofected ADMSCs as analyzed by quantitative RT-PCR and ELISA respectively .